85-1448-73　［取扱停止］CD152, Fc Chimera, Recombinant, Human (CTLA4, Cytotoxic T-lymphocyte-associated Antigen 4) 25ug　C2548-83S

特徴

• CTLA-4 is expressed in low copy number by T-cells only after activation, but it binds CD28-ligand with approximately 20-fold higher affinity than CD28. A soluble form of the extracellular domain of CTLA-4 has been shown to bind CD28-ligand with high avidity and to suppress T-cell-dependent antibody responses in vivo. Large doses of this soluble protein also suppress responses to a second immunization. Recombinant Human CTLA-4 produced in insect cells is a homodimeric, glycosylated, polypeptide chain containing a total molecular mass of 80kDa. Each subunit (40kDa) is fused to a polypeptide linker to the Fc portion of Human IgG1. CTLA-4 is expressed in low copy number by T-cells only after activation, but it binds CD28-ligand with approximately 20-fold higher affinity than CD28. A soluble form of the extracellular domain of CTLA-4 has been shown to bind CD28-ligand with high avidity and to suppress T-cell-dependent antibody responses in vivo. Large doses of this soluble protein also suppress responses to a second immunization. Recombinant Human CTLA-4 is purified by proprietary chromatographic techniques.
• Amino acid sequence:The sequence of the amino acids was determined and was found to be: Lys-Ala-Met-His-Val.
• Dimers and aggregates:≤ 1% as determined by silver-stained SDS-PAGE gel analysis.
• Biological Activity:Recombinant CTLA-4 is fully biologically active when compared to standards.
• Protein content:Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280. 2. Analysis by RP-HPLC, using a standard solution of Human CTLA-4 as a Reference Standard.
• Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile ddH2O, 0.1% HSA or BSA. aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer