85-1448-39　［取扱停止］Calpain I (Mu Calpain, Protein Kinase C Activating Factor) 5ug　C1035-26Z

特徴

• This protein consists of an 80kD large subunit and a 30kD small subunit. The protein was purified by sequential chromatography through DEAE-Sepharose, A1.5m Bio-Gel, and Phenyl-Sepharose CL-4B columns.
• Applications:This protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection.
• For adsorption we suggest the following procedure: A- Dilute 1µl of the antiserum against µ-calpain in 1ml of the usual buffer for immunohistochemistry (final dilution 1:1000). B- Add 1µg of protein to 1ml of the diluted antibody solution and mix well. C- Incubate for at least 6 hours in the cold. D- Apply to tissue-sections and incubate for 3 days. E-Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex, DAB).
• As a result, the immunostaining should be strongly reduced or even completely prevented.
• Stability:µ-Calpain although stable at 100C for 1 week, should be stored desiccated below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.