85-1447-80　［取扱停止］Alkaline Phosphatase, Bovine (AP) 1mg　A1355-65B

特徴

• Alkaline Phosphatase is Dimeric protein having a molecular weight of 140kD, one Zn++ ion is tightly bound to each subunit, and another less tightly bound is involved in the catalytic reaction. Mg++ stimulates the catalysis. The binding site for Mg++ is different to that of Zn++, but will be occupied by excess Zn++ followed by loss of enzyme activity. Alkaline Phosphatase is purified by affinity chromatography, which results in an enzyme of high specific activity and purity.
• Activity:~1000U/mg (pH 9.6, 37°C, with 0.025M glycine, 10% glycerol as buffer).
• Storage and Stability:May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

仕様

• Size：1mg
• Source Antigen：Calf Intestine
• Grade：Highly Purified
• Purity：≥ 95% pure by Gel Filtration.
• Form：Supplied as a liquid in 5mM MgCl2, 0.1mM ZnCl2, 5mM TRIS, pH 7.0, 50% glycerol.
• Molecular Weight Non Ab：140
• EU Commodity Code：38220090
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