Assay Condition:The kit performance has been optimized for the stated protocol using the materials listed and standard dilutions from 0.27-200ng/ml of human Haptoglobin. For alternative assay conditions, the operator must determine appropriate dilutions of reagents. ELISA assay reactivity is sensitive to any variation in operator, pipetting and washing techniques, incubation time or temperature, composition of reagents, and kit age. Adjustments may be required to position the standard curve and/or samples in the desired detection range.
Specificity:By immunoelectrophoresis and ELISA the antibodies in this kit react specifically with human Haptoglobin, not with other human serum proteins. The following factors prepared in the detection range of this kit, 0.27-200ng/ml were assayed and exhibited no cross-reactivity or interference: Human Serum Albumin, Human Transferrin, Human IgG.
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
