This ELISA kit is intended for the quantitative determination of human Fibrinogen levels in serum or other biological samples.
Kit Components:Complete ELISA kit with solid phase antibody coated on the plate and all the other required reagents provided
F4202-02A Coating Antibody 1x1vial, affinity-purifed chicken anti-human F4202-02B Calibrator 1xvial, Pure Human Fibrinogen AntigenF4202-02C Detection Antibody (HRP) 1x1vial, Supplied as an affinity-purified Chicken anti-Human Fibrinogen (HRP)
Range of Detection: 2000-2.7ng/ml
Storage and Stability:Store F4202-02C at 4°C. Aliquot other components into 10 vials and store at -20°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Assay Condition: The kit performance has been optimized for the stated protocol using thematerials listed and standard dilutions from 2000-2.7ng/ml of human Fibrinogen. For alternative assay conditions, the operator must determine appropriate dilutions of reagents. ELISA assay reactivity is sensitive to any variation in operator, pipetting and washing techniques, incubation time or temperature, composition or reagents, and kit age. Adjustments may be required to position the standard curve and/or samples in the desired detection range.
Specificity: By immunoelectrophoresis and ELISA the antibodies in this kit react specifically withhuman Fibrinogen, not with other human serum proteins. The following factors prepared in the detection range of this kit, 2000-2.7ng/ml were assayed and exhibited no cross-reactivity or interference: Human Serum Albumin, Human Transferrin, Human IgG
