Human Akt1 (GenBank Accession No. NM_001014432), (a.a. 1-480) with N-terminal HIS6 tag, MW 60.2kD, expressed in an Baculovirus infected Sf9 cell expression system.
Application: useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Source: Baculovirus infected Sf9 cells
Specific Activity: 100U/mg. One unit is defined as the amount of enzyme that will phosphorylate 1nmol of Crosstide substrate per minute at pH 7.4 and 30°C.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
仕様
Size:100ug
Source Antigen:Recombinant, human from Baculovirus infected Sf9 cells
Grade:Purified
Purity:≥80% by SDS-PAGE.
Form:Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM NaCl, 0.05% Tween-20, 50% glycerol, 3mM DTT.