Tie-1 (human 1138 aa; mouse 1134 aa) is a type 1 membrane receptor protein specifically expressed in developing vascular endothelial cells. Murine and human Ang-1 are 498aa secreted angiogenic proteins. It is only 60% homologous with Ang-2. Ang-1 is the natural ligand for Tie-2 and is primarily expressed in developing endothelial cells.
Source:Mouse myeloma cell line, NS0-derived recombinant protein corresponding to human Angiopoietin-1, Ser20-Phe498, with a C-terminal 6-His tag
Molecular Mass: Based on N-terminal sequencing, the recombinant human angiopoietin-1 protein starts at Ser 20 and has a calculated molecular mass of 56kD. As a result of glycosylation, the recombinant protein migrates as an approximately 70kD band in SDS-PAGE under reducing conditions.
Biological Activity:Measured by its ability to inhibit serum deprivation induced apoptosis in human umbilical vein endothelial cells (HUVEC) (6). The ED50 for this effect is typically 10-40ng/ml in the presence of 5ug/ml of a cross-linking antibody (mouse anti-6X histidine).
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：25ug
Source Antigen：Recombinant, NSO cells
Grade：Purified
Purity：≥90% (SDS-PAGE and visualized by silver stain). Endotoxin: ≤1EU/ug (LAL)
Form：Supplied as a lyophilized powder from PBS. Reconstitute with 250ul sterile PBS.
