Tumor Necrosis Factor alpha (TNF-alpha) is a 17.3kD multi-functional pro-inflammatory cytokine primarily secreted by macrophages, but also produced by T and B lymphocytes, activated monocytes and fibroblasts. TNF-alpha exists in both a transmembrane and mature soluble form and has many biological properties, including activation of transcription factor NF-KappaB, the induction of apoptosis in various tumor cell lines and the stimulation of cell differentiation and proliferation under certain conditions. Studies have revealed a role for TNF-alpha in inflammation, septic shock, autoimmune disease and rheumatoid arthritis.
Source:Recombinant corresponding to rat Tumor Necrosis Factor alpha expressed in E. coli.
Endotoxin: ~0.1ng/ug
Biological Activity:ED50 range =0.005-0.02ng/ml, determined by the dose dependent cytolysis of mouse L-929 cells in the presence of actinomycin D. Optimal concentration for individual application should be determined by a dose response assay.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：~95% (SDS-PAGE). Purified by sequential chromotography.
Form：Supplied as a lyophilized powder. BSA free. Reconstitute with sterile dH2O to 0.1-1mg/ml.
