TGF-B is a multifunctional polypeptide growth factor. While specific receptors for this protein have been found on almost all mammalian cell types, the effect of the molecule varies depending on the cell type and growth conditions. Generally, TGF-B is stimulatory for cells of mesenchymal origin and inhibitory for cells of epithelial or neuroectodermal origin. It is a family of regulatory proteins consisting of a number of proteins distantly related. TGF-B1 has been found in the highest concentration in human platelets and mammalian bone, but is produced by many cell types in smaller amounts. TGF-B2 has been found in the highest concentration in porcine platelets and mammalian bone, but again is also produced by many types of cells. The heterodimer, TGF-B1.2, has so far been found only in small amounts in porcine platelets. TGF-B3 has been detected in human, porcine, and avian sources, mainly in cells of mesenchymal origin, suggesting a different role for this protein than for TGF-B1 or TGF-B2. TGF-B4 has been detected so far only in chick embryo chondrocytes, and its distribution in other types of cells has not yet been characterized. TGF-B5 has been detected only in Xenopus embryos. TGF-B1, TGF-B2, and TGF-B1.2 appear to be largely equivalent in biological activity, although there appear to be differences in binding to certain types of receptors, and there are a few reports of differential responses to TGF-B1 and TGF-B2.
Source:Recombinant corresponding to 112aa from human Transforming Growth Factor beta2 expressed in E. coli.
Molecular Weight:~25kD
Endotoxin: ~0.1ng/ug
Biological Activity:ED50 ≤0.2ng/ml (≥5x10e6units/mg), determined by its ability to inhibit the mouse IL-4 dependent proliferation of mouse HT-2 cells. TGF-b2 has growth inhibitory action and regulates the proliferation and differentiation of normal and transformed cells. Differential expression of TGF isoforms is observed in certain disease states. Human PC-3 prostatic carcinoma cell line and human glioblastoma cell lines predominantly secrete the TGF-b2 isoform over the other isoforms.
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：5ug
Source Antigen：Recombinant, Human from Baculovirus infected High-5 cells
Grade：Highly Purified
Purity：~98% (SDS-PAGE, HPLC). Purified by sequential chromatography.
Form：Supplied as a lyophilized powder from sodium citrate buffer, pH 3.5. Sterile filtered prior to lyophilization. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml. Do not vortex.
