Tau, Total, Human (Tau Protein, Microtubule-associated Protein, MAP) BioAssay™ ELISA Kit is used for the quantitative determination of human total Tau protein. Tau is a microtubule associated protein found predominantly in neuronal axons of vertebrate brain. Tau protein is highly soluble and normally attached to axonal microtubules. Tau stabilizes the microtubules and makes them rigid. Tau interacts with actin in the cytoskeleton and neuronal outgrowth, anchors enzymes such as protein kinases and phosphatases, and regulates intracellular vesicle transport. Tau phosphorylation regulates both normal and pathological functions of this protein. Tau, in its hyperphosphorylated form, is the major component of paired helical filaments (PHFs), the building block of neurofibrillary lesions in Alzheimer’s disease (AD). Hyperphosphorylation impairs the microtubule binding function of tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to neuronal degeneration. Deposition of filamentous tau is implicated in other neurodegenerative diseases including cortical basal degeneration (CBD), progressive supranuclear palsy (PSP), Pick’s disease and certain forms of Parkinson’s disease.
Sensitivity:<10pg/ml
Range:31.2-2000pg/ml
Incubation Time:4 hours
Sample Size: 50ul
Accuracy:Inter-assay CV: ≤10%Intra-assay CV: ≤8%
Kit Components:T1034-25A: Microtiter Plate 1x96 wells Coated with human Tau antibodyT1034-25B: Standard 2x1 vial T1034-25C: Standard Diluent Buffer. 1x25ml T1034-25D: Detection Antibody 1x11ml T1034-25E: Pab (HRP), 100X 1x125ulT1034-25F: HRP Diluent 1x25mlT1034-25G: Wash Buffer, 25X 1x100mlT1034-25H: TMB 1x25ml T1034-25J: Stop Solution 1x25ml
Storage and Stability:Store all components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
