Source:Recombinant corresponding to mouse Platelet Derived Growth Factor BB expressed in E. coli.
Molecular Weight:~25kD
Endotoxin: ~0.1ng/ug
Biological Activity:ED50 range=1-5ng/ml, determined by the dose-dependent proliferation of BALB/c 3T3 cells. The optimal concentration for each specific application should be determined by an initial dose-response assay.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 100mM acetic acid, 0.1% BSA, aliquot and store at ≤- 20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：~95% (SDS-PAGE). Endotoxin: ≤ 0.1ng/ug.
Form：Supplied as a lyophilized powder, carrier free. Reconstitute in 100mM acetic acid, 0.1% BSA to 0.1-1mg/ml to regain full activity. Further dilutions should be made in low endotoxin medium or buffered solution with FBS or tissue culture grade BSA.
