Source:Recombinant corresponding to human Platelet Derived Growth Factor AA expressed in E. coli.
Molecular Weight:~28.5kD
Endotoxin: ~0.1ng/ug
Biological Activity:ED50 range=1-3ng/ml, determined by the dose-dependent proliferation of BALB/c 3T3 cells. The optimal concentration for each specific application. Platelet derived growth factor is a mitogen for a variety of cell types, including connective tissue, smooth muscle tissue and fibroblasts.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile ddH2O, 0.1% BSA, aliquot and store at-20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size:10ug
Source Antigen:Recombinant, E. coli
Grade:Highly Purified
Purity:~98% (SDS-PAGE, HPLC)
Form:Supplied as a lyophilized powder, carrier free. Sterile filtered prior to lyophilization. Reconstitute in sterile ddH2O, 0.1% BSA to 0.1-1mg/ml.