Molecular Weight:35kD (migrates at ~48kD on SDS-PAGE gel)
Purity:≥95% as determined by SDS-PAGE analysis. Purified by sequential chromatography.
Biological Activity:Recombinant human NFAT (Nuclear Factor of Activated T-cells) is phosphorylatable in vitro, using recombinant GSK-3β and PKA. Optimization of the cell stimulation protocol, cell lysis procedure and reaction conditions may be required for each specific application.
Source:Recombinant human NFATc1 (amino acid residues 2-298) is produced in E. coli with an amino-terminal polyhistidine tag.
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：25ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：≥95% as determined by SDS-PAGE analysis. Purified by sequential chromatography.
