Fibroblast growth factor 4 is a member of the FGF family, a group of 7 homologous polypeptides that act upon a variety of cells and produce biological actions that may include cell proliferation, migration and differentiation. FGF4, also known as oncogene products hst1 and KFGF, has strong transforming potential and is a potent angiogenic factor, being expressed in several highly vascularized tumors.
Source:Recombinant corresponding to 182aa from human Fibroblast Growth Factor 4 expressed in E. coli.
Molecular Weight:~19.7kD
Endotoxin: ≤0.1ng/ug
Biological Activity:ED50 ≤0.5ng/ml (≥2x10e6 units/mg), determined by the dose dependent stimulation of thymidine uptake by BaF3 cells expressing FGF-receptors. FGF-4 is a heparin binding growth factor expressed by tumor cells and embryonic cells which has mitogenic activity with fibroblasts and endothelial cells.
Applications:Suitable for use in Neutralization. Other applications not tested.
Recommeded Dilutions:Optimal dilution to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C with a carrier protein. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：25ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：Purified by sequential chromatography. Tested by SDS-PAGE, HPLC (≥95%)
Form：Supplied as a lyophilized powder from 10mM sodium phosphate, pH 7.4, 100mM sodium chloride. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml. Do not vortex. Further dilute for extended storage with a buffer containing a carrier protein (0.1% BSA or HSA).
