Islet-activating protein Pertussis toxin consists of an A protomer subunit (S1) which possesses both NAD+ glycohydrolase and ADP-ribosyltransferase activities, and B oligomer subunits (S2, S3, S4, and S5) which are responsible for attachment of the native toxin to eukaryotic cell surfaces. Pertussis toxin uncouples G proteins from receptors by ADP ribosylating a cysteine residue near the carboxyl terminus of the a subunit.
This preparation migrates as five distinct bands when analyzed by SDS-Urea PAGE. The five bands correspond to one A protomer subunit, designated S1 (Mr=26.2kD), and four B oligomer subunits, designated S2-S5 (Mr’s= 21.9, 21.9, 12.1, and 10.9kD, respectively).
Reconstitution:Reconstitute with 500ul sterile ddH2O. Because Pertussis toxin is relatively insoluble, the resulting suspension should be made uniform by gentle mixing prior to withdrawing aliquots. Do not sterile filter.
This preparation is not activated. For use with intact cells or extracts, activation is not necessary. However, when used with purified G proteins, this preparation should be activated according to the procedure of Kaslow et al., 1987.
Biological Activity:The lowest concentration which produces a clustered growth pattern with CHO cells is 0.03ng/ml. The adenylate cyclase activity of this preparation is 20.1 picomoles/minute/ug in the presence of 1ug calmodulin.
Storage and Stability:Lyophilized powder may be stored at 4°C. Stable for 6 months after receipt at 4°C. Reconstitute with sterile ddH2O. For long-term storage, aliquot and store at 4°C. Do not freeze. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Bordetella pertussis
Grade：Highly Purified
Purity：~99% (SDS-PAGE)
Form：Supplied as a lyophilized powder from 10mM sodium phosphate, pH 7.0, 50mM sodium chloride. Reconstitute with 500ul sterile ddH2O.
