85-1444-10　［取扱停止］Amyloid Precursor Protein, Human (APP) BioAssay™ ELISA Kit 96Tests　A2275-69L

特徴

• Sensitivity:<0.4ng/ml The analytical sensitivity of this assay is <0.4ng/ml of Human APP. This was determined by adding two standard deviations to the mean O.D. obtained when the zero standard was assayed 30 times.
• Specificity:The Human APP BioAssay™ ELISA kit is specific for measurement of total APP protein. The following proteins were tested at 100ng/ml and found to have no crossreactivity: Tau, a-synuclein, b-synuclein, Ab1-40 and Ab1-42.
• Range: 0.78-50ng/ml
• Incubation Time:4 hours
• Sample Size:10ul (diluted to a total volume of 100ul)
• Sample Type:Cell lysates and cerebral spinal fluids
• Specifications:Simple: Pre-coated strip-well plates
• Liquid stable chromogen and stop reagents
• Economical: One 96-well plate, plus reagents for 96 determinations
• Reagents are stable for multiple runs.
• Precision:Intra-assay: <8%Inter-assay: <9%
• Kit Components:*A2275-69L1: Standard, 2x1 vialSupplied as a lyophilized powder. Reconstitute with 1ml of A2275-69L2: Standard Diluent Buffer
• A2275-69L2: Standard Diluent Buffer,1x25mlSupplied as a liquid containing 8mM sodium azide
• A2275-69L3: Microtiter Plate, 1x96 wells A2275-69L4: anti-APP (Biotin), 1x11mlA2275-69L5: Streptavidin (HRP) (100X), 1x125ulSupplied as a liquid containing 1.7mM thymol
• A2275-69L6: Streptavidin (HRP) Diluent, 1x25mlSupplied as a liquid containing 1.7mM thymol and 0.05% Proclin 300
• A2275-69L7: Wash Buffer (25X), 1x100mlA2275-69L8: TMB, 1x25mlA2275-69L9: Stop Solution, 1x25ml
• Storage and Stability:Store *A2275-69L1: Standard powder at 4°C, liquid at -70°C. Store other components at 4°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.