Streptavidin is a non-glycosylated polypeptide chain having a molecular mass of 240kD and containg 4 subunits each with a binding site for biotin. The neutral isoelectric point of Streptavidin and the high affinity of the biotin-streptavidin interaction result in a very quick reaction with a high signal to noise ratio. Streptavidin does not react non-specifically with endogenous lectins when used in immunological assays on cells and tissues. Streptavidin is non- or very low charged around pH 7, reducing NSB to charged molecules to a very limited level.
Applications:Suitable for use in ELISA, Immunoblot (Western and Dot Blot), Immunohistochemistry and Immunoperoxidase Electron Microscopy. Other applications not tested.
Recomended Dilution:ELISA: 1-2ug/mlWestern Blot: 0.01-0.1ug/ml for Western blotting with ECL substrates1-2ug/ml for Western blotting with chromogenic substratesImmunohistochemistry: 1-2ug/mlOptimal dilutions to be determined by the researcher.
Reconstitution and Storage:Store freeze-dried product at 2-8°C until reconstitution. When ready to use, reconstitute with 1 ml di water. Centrifuge product if the solution is not clear after reconstitution. To judge clarity, draw product into a clean pasteur pipette. Storing the product for more than one day at final working dilutions is not recommended. For extended storage as a liquid, add an equal volume of glycerol (ACS or better grade) for a final concentration of 50% followed by storage at -20°C. Please note that the concentration of protein and buffer salts will be one-half of the original after the addition of glycerol. Alternatively, the product may be aliquoted and frozen at -70°C or below, in the absence of glycerol. It is important to avoid repeated freezing and thawing.
Expiration Date: one year from date of reconstitution.
Country of Origin: USA
仕様
Size：1mg
Source Antigen：Streptomyces avidinii
Grade：Highly Purified
Purity：Purified by ion exchange chromatography.
Form：Supplied as a lyophilized powder in 0.01M sodium phosphate, 0.25M sodium chloride, pH 7.6, 15mg/ml BSA (IgG and Protease Free). No preservative added. Labeled with horseradish peroxidase (HRP). (Note: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.)
Specificity：Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase and anti-streptavidin. No reaction was observed against anti-avidin.
