Myostatin (GDF-8) is expressed uniquely in human skeletal muscle as a 12kD mature glycoprotein consisting of 113aa and secreted into plasma. Myostatin is a member of the transforming growth factor beta superfamily of secreted growth and differentiation factors that is essential for proper regulation of skeletal muscle mass. Studies have shown that myostatin could play an important role in cardiac development and physiology.
Total 152AA. M.W. 16.7kD (calculated). N-terminal His-tag and spacer (43AA – highlighted). The AA sequence of the human myostatin part of the fusion protein is corresponding to the UniProtKB/Swiss-Prot entry O14793
Recombinant Human Myostatin part of the fusion protein is corresponding to amino acid sequence of the human Myostatin (Acc. No. AF019627, R263-S376).
Applications:Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：Human, recombinant expressed in E. coli.
Grade：Affinity Purified
Purity：≥ 95% by SDS-PAGE.
Form：Supplied as a lyophilized powder from 0.05M acetate buffer, pH 5.5. Reconstitute in sterile 0.1M acetic acid, pH4.0 to ~0.5mg/ml. Let pellet dissolve completely.
