The peptide Polymyxin B (PMB) is a cyclic amphipathic peptide antibiotic that binds to endotoxins (lipopolysaccharide, LPS). Binding of PMB to LPS results in neutralization of LPS of gram-negative bacteria. For the study of PMB-LPS interactions PMB conjugates proved to be superior to the application of anti-PMB antibodies. PMB conjugates are useful general probes to detect or measure lipid A and LPS of various species using simple methods. These conjugates are also useful for staining of bacteria. The conjugates obviate the need for many specific antisera. Furthermore PMB conjugates are useful tools in investigations of LPS binding protein (LBP) and bactericidal permeability increasing protein (BPI) interactions with LPS. In conclusion, PMB conjugates are useful probes for endotoxin research.
Applications:Suitable for use in ELISA. Other applications not tested.
Recommended Dilutions:ELISA: 1:50Optimal dilution determined by the researcher
Storage and Stability:Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：200ul
Grade：Purified
Purity：Purified
Form：Supplied as a lyophilized powder from PBS, 0.1% BSA and 0.02% 2-chloroacetamide. Labeled with Biotin. Reconstitute with 200ul sterile ddH2O.
