Source:Recombinant protein expressed in Pichia pastoris
Description:Bovine Tumor Necrosis Factor alpha (TNF alpha) is produced by a range of cells, including macrophages, monocytes, T and B lymphocytes. TNF alpha is an important mediator of the bovine inflammatory response.
Biological Activity:ED50 in the range of 10-25ng/ml, determined by measuring the cytolytic effect of recombinant bovine TNF alpha on murine WEHI-13VAR cells in the presence of Actinomycin D.
Endotoxin Level:<0.06EU/ug.
Applications:Suitable for use in In ELISA and Functional assays. Other applications not tested.
Storage and Stability:Lyophilized and reconstituted products may be stored at -20°C. Stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：20ug
Source Antigen：Recombinant, Pichia pastoris
Grade：Highly Purified
Purity：≥95% (SDS PAGE)
Form：Supplied as a lyophilized powder in PBS. No preservative added. Reconstitute with 500ul sterile ddH2O.
