Equine Tumor necrosis factor alpha (TNF alpha) is produced by a range of cells, including macrophages and monocytes and is an important mediator of the equine inflammatory response.
Recombinant protein corresponding to equine TNFa, expressed in Pichia pastoris.
Biological Activity:Determined by measuring the cytolytic effect of recombinant equine TNF alpha on murine WEHI-13VAR-164 cells in the presence of Actinomycin D the ED50 is <0.05ng/ml.
Applications:Suitable for use in In vitro applications. Other applications not tested.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：20ug
Source Antigen：Recombinant, Pichia pastoris
Grade：Highly Purified
Purity：~95% (SDS PAGE). Endotoxin: 0.15EU/ug
Form：Supplied as a lyophilized powder. Reconstitute with 500ul sterile ddH2O.
