Streptavidin (MW ~66kD) binds specifically with biotin (244D). It is derived from the bacterium Streptomyces avidinii and bears a remarkable similarity to chicken egg-white avidin both in three-dimensional structure and its ability to bind biotin with extremely high affinity (Kd=10e-15M). Streptavidin is a tetrameric protein capable of binding up to 4 biotin molecules. Unlike avidin, Streptavidin is non-glycosylated and is essentially neutral in charge, whereas avidin (pH 10.5) is basic at neutral pH. Because of this, streptavidin has considerably less non-specific binding that results in less background. It has replaced avidin as the reagent of choice for most applications where protein interactions may cause background.
Applications:Suitable for use in Immunofluorescence, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilutions:Immunofluorescence:1:20-1:50 Immunohistochemistry: 1:20-1:50Immunocytochemistry: 1:20-1:50Dilutions to be made with PBS, 10% normal goat serum
Optimal dilutions to be determined by the researcher.
Absorption: 552nm
Emission:565nm
Storage and Stability:May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. CY3 conjugates are sensitive to light.
仕様
Size：1mg
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide, 40% glycerol. Labeled with CY3.
