Avidin/Biotin Blocking Kit BioAssay™ is designed to eliminate background caused by endogenous avidin or biotin-like substances. The kit contains 18ml each of avidin and biotin solutions.
Kit Components:A4500-14A: Avidin Solution, 1x18ml. Supplied as a liquid, 0.05% sodium azideA4500-14B: Biotin Solution, 1x18ml. Supplied as a liquid, 0.05% sodium azide
Protocol:These blocking steps should be performed before the primary antibody incubation step
1. Apply 100ul (2 drops) of A4500-14A to the tissue
2. Incubate for 10 minutes at RT
3. Rinse extensively with PBS
4. Apply 100ul (2 drops) of A4500-14B to the tissue
5. Incubate for 10 minutes at RT
6. Rinse extensively with PBS
The endogenous avidin/biotin activity is now blocked and the tissue section is ready for immunohistochemical staining.
Storage and Stability:Store components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
