Protein A (from Staphylococcus aureus, Cowan I Strain) is conjugated with highly purified horseradish peroxidase (RZ > 3.0).
Applications:Suitable for use in Immunoblot (Western or Dot Blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions:Western Blot: 1:2000-1:10,000Dot Blot: 1:2000-1:10,000ELISA: 1:20,000-1:100,000Immunohistochemistry: 1:1000-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder is stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O prior to use. To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1mg
Source Antigen：Staphylococcus aureus, Cowan I Strain
Grade：Highly Purified
Purity：Purified by chromatography prior to conjugation.
Form：Supplied as a lyophilized powder from 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 10mg/ml BSA, and 0.01% gentamicin sulfate. Labeled with HRP. Rehydration: Add 500ul sterile, distilled water to make a 2mg/ml stock solution.
