Pervanadate is a protein tyrosine phosphatase inhibitor that is commonly used to increase tyrosine phosphorylation in cells. Human aortic endothelial cells treated with pervanadate for 30 minutes display a significant increase in tyrosine phosphorylation as shown by westernblotting using anti-Phosphotyrosine. Confluent cultures of human aortic endothelial cells were serum starved overnight. Cells were then either left untreated or treated with pervanadate (1mM) for 30 minutes at 37°C. Cells were lysed in 1% SDS, 1mM sodium ortho-vanadate,10mM Tris (pH 7.4) buffer. Protein concentration was determined using the BCA method (Pierce) before diluting to final concentration and buffer.
Applications:SDS-PAGE/Western blotting 20ml/well
End user should determine optimal quantity for their particular applications and experiments.
仕様
Size：100ug
Source Antigen：Human aortic endothelial cells
Grade：Lysate
Form：Supplied in electrophoresis sample buffer (62.5mM Tris pH 7.2, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% b-mercaptoethanol).
