Control Peptide for C2069-54 Catenin, beta Pab RbxHu
b-Catenin is a 92kD protein that binds to the cytoplasmic tail of E-Cadherin. The cadherins, transmembraneadhesion molecules, are found with catenins at adherens junctions. Deletions in the cytoplasmic domain of E Cadherin eliminate catenin binding and result in a loss of cell adhesion. Tyrosine phosphorylation of b-Catenincan regulate its interaction with critical components of adherens junctions. Both Fer and Fyn kinases phosphorylate tyrosine 142 in vitro. Overexpression of these kinases in epithelial cells disrupts interactions between a- and b-Catenins. The phosphorylation of tyrosine 142 may act as a switch from the transcriptional to the adhesive role of b-Catenin. Src family kinases can also phosphorylate tyrosine 654 in the C-terminal armadillo repeat of b-Catenin. This phosphorylation regulates b-Catenin binding to E-cadherin. Thus, sitespecifictyrosine phosphorylation of b-Catenin may regulate specific protein-protein interactions leading to changes in cell adhesion.
Applications:Suitable for use in ELISA and for blocking antibody reactivity in Western Blot and Immunocytochemistry. Other applications not tested.
Recommended Dilution:ELISA: 10-100ng/well. (Peptides were established in 96-well plates where the peptide was bound to plates for 2 hrs in 0.1 M sodium carbonate buffer, pH 8.5.)Optimal dilution to be determined by the researcher
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Grade：Purified
Purity：Purified.
Form：Supplied as a liquid in PBS with 0.05% sodium azide.
