Full length Recombinant SIV gp130 glycoprotein produced in the Baculovirus Expression System.
Specificity:This protein binds to murine monoclonal antibodies of defined epitope specificity and simian serum polyclonal antibodies in ELISA and Western Blot.
Biological Activity:Binds to human T-cell receptor CD4 in ELISA and Western Blot as determined by CD4/gp130/Anti-gp130 mAb-peroxidase capture ELISA. Activates simian T-Lymphocytes (CD4+, CD4-), in vitro, as measured by RNA synthesis during G0 to G1 transition phase of antigen-binding competent cells.
Applications:Suitable for use in CD4 Binding, B and T cell Activation, ELISA and Western Blot. Also suitable for immunization. Other applications not tested.
Recommended Dilutions:ELISA: 10-100ng Western Blot: 10-100ng Optimal dilutions to be determined by the researcher.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Source Antigen：Recombinant, Baculovirus
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography to ≥90% (SDS-PAGE, reduced).
Form：Supplied as a liquid in PBS, pH 7.2. No stabilizers or preservatives added.
