Full length Recombinant HIV-1 HXB2 vif produced as a 6xHis-fusion protein in E. coli.
Specificity:This protein binds to murine monoclonal antibodies and HIV-1 converted human serum polyclonal antibodies in ELISA and Western Blot
Biological Activity:Vif dependent HIV-1 virus production in HIV-1 infected SupT1, Jurkat and CEM cells.
Applications:Suitable for use in ELISA, Western Blot, Drug screening and Immunological studies. Other applications have not been tested.
Recommended Dilutions:ELISA: 1-10ngWestern Blot: 1-10ng Optimal dilutions to be determined by the researcher.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ug
Source Antigen:Recombinant, E. coli
Grade:Affinity Purified
Purity:Purified by Nickel-affinity chromatography and the purified protein is cleaved to produce vif of ≥99% purity, as determined by SDS-PAGE, reduced
Form:Supplied as a liquid in 50mM Tris, pH 8.0, 150mM sodium chloride. No stabilizers or preservatives added.