Coupling Ratio:~10-20 moles of biotin per mole tat.
Biological Activity:Biotin tat binds to solid phase murine anti-tat mAb and human rHexim1 as determined by capture ELISA using strepavidin-HRP as a secondary reagent.
Applications:Suitable for use in T Cell Research, ELISA and Western Blot. Other applications not tested.
Recommended Dilutions:A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. 1. ELISA may be performed in 1:1000 dilution range depending on the concentration of the test ligand and specificity and sensitivity of the secondary reagents. tat/anti-tat mAb capture ELISA may be performed in 100ng mAb in solid phase and 1-10ng of biotin-tat.
Storage and Stability:May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Recombinant, E. coli
Grade：Affinity Purified
Purity：Purified by ion affinity and reverse phase HPLC to ≥ 99% as determined by SDS-PAGE and HPLC.
Form：Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.01% Thimersol. Labeled with Biotin.
