Molar Ratio:2-4 peroxidase molecules per molecule of tat.
Specificity:Peroxidase conjugated tat binds to murine monoclonal antibodies of defined epitope specificity and rabbit polyclonal antibodies in ELISA and Western ELISA.
Applications:A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. 1. ELISA and Western ELISA may be performed at dilutions of 1:500 range depending on the concentration of antibody in the test system.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:200Tests
Source Antigen:Recombinant, E. coli
Grade:Affinity Purified
Purity:Purified by ion affinity and reverse phase HPLC to ≥ 99% as determined by SDS-PAGE and HPLC.
Form:Supplied as a liquid in 10mM Tris, pH 8.0, 2.5% BSA, 0.01% thimerosal. Labeled with HRP.