p17 HIV-1 IIIB (E. coli) conjugated to Colloidal Gold (25nm mean particle size).
Coupling Ratio:OD520nm = 10; protein content approximately 25ug.
Specificity:Gold conjugated p17 binds to murine monoclonal antibodies of defined epitope specificity and human polyclonal antibodies as determined by dot blot assays. Reaction time: 5-10 minutes at room temperature.
Applications:A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. 1. Dot blot assays may be performed in 1:100 dilution range depending on the concentration of solid phase Anti-p17 in the test system. 2. Immunohistochemical studies may be performed in 1:50 dilution range.
Storage and Stability:May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. Gold conjugates are light sensitive.
仕様
Size：1ml
Source Antigen：Recombinant, E. coli
Grade：Affinity Purified
Purity：Purified by GST-agarose affinity chromotography and p17 is cleaved from the fusion protein by using Factor Xa. GST is removed from the mixture by a second passage through GST-agarose.
Form：Supplied as a liquid in 10mM Tris, pH 8.0, 5% BSA, 0.05% PEG, 0.02% sodium azide. Labeled with Colloidal Gold.
