Recombinant HIV-1 p17 is expressed as a Glutathione S-transferase (GST) fusion protein produced in an E. coli expression system.
Specificity:This protein binds to HIV-1 positive human serum polyclonal antibodies in ELISA and Western Blot and murine monoclonal antibodies of defined epitope specificity.
Biological Activity:Recombinant p17 core protein contains authentic N- and C-termini which are normally processed from the sequence of the matrix antigen with relevant HIV-1 protease.
Applications:A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. Dilute p17 stock solution in MES buffer immediately before use. ELISA assays may be performed with p17 in solid phase in 10-100ng range. Western Blotmay be performed in 100-500ng depending on the specificity of the primary antibody used in the test system.
Storage and Stability:For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.
仕様
Size：100ug
Source Antigen：Recombinant, E. coli
Grade：Affinity Purified
Purity：Purified by GST-agarose affinity chromotography and p17 is cleaved from the fusion protein by using Factor Xa. GST is removed from the mixture by a second passage through GST-agarose.
Form：Supplied as a liquid in MES Buffer, pH. 6.5. No stabilizers or preservatives added.
