Coupling Ratio:~5-10 moles of biotin per mole nef.
Biological Activity:Binds to solid phase murine anti-nef monoclonal antibodies as determined by capture ELISA using strepavidin-HRP as a secondary reagent.
Applications:Suitable for use in T-Cell Research, ELISA and Western ELISA. Other applications not tested.
Recommended Dilutions:ELISA: 1:1000Capture ELISA: 1-10ngOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
仕様
Size：50ug
Source Antigen：Recombinant, E. coli
Grade：Affinity Purified
Purity：Purified by glutathione affinity chromatography and the purified protein is thrombin-cleaved to produce nef of ≥95% purity, as determined by SDS-PAGE, reduced.
Form：Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.09% sodium azide. Labeled with Biotin.
