Full length recombinant protein corresponding to HIV-1 IIIB gp120 glycoprotein produced in the CHO Expression System.
Biological Activity:This protein binds to human T-cell receptor CD4 as determined by CD4/gp120/Anti-gp120 mAb-peroxidase capture ELISA. This protein activates human T-Lymphocytes (CD4+, CD4-), in vitro, as measured by RNA synthesis during G0 to G1 transition phase of antigen-binding competent cells.
Applications:Suitable for use in ELISA and Western ELISA. Also suitable for use in CD4 binding and T-cell activation studies. Other applications have not been tested.
Recommended Dilutions:ELISA: 10-100ngWestern Blot: 10-100ngOptimal dilutions to be determined by the researcher.
Storage and Stability:For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:50ug
Source Antigen:CHO cells
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography to ≥95% purity as determined by SDS-PAGE, reduced.
Form:Supplied as a liquid in PBS, pH 7.2. No stabilizers or preservatives added.
Specificity:This protein binds to murine monoclonal antibodies of defined epitope specificity and human serum polyclonal antibodies in ELISA and Western ELISA.