rgp160 HIV-1 IIIB conjugated to Colloidal Gold (25nm mean particle size).
Coupling Ratio:OD520nm = 10; protein content approximately 25ug.
Specificity:Gold conjugated rgp160 binds to murine monoclonal antibodies of defined epitope specificity and human/rabbit polyclonal antibodies as determined by dot blot assays.
Applications:A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. 1. Dot blot assays may be performed in 1:100 dilution range depending on the concentration of solid phase CD4/Anti-gp160 in the test system. 2. Immunohistochemical studies may be performed in 1:50 dilution range.
Storage and Stability:May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. Gold conjugates are light sensitive.
仕様
Size:1ml
Source Antigen:Recombinant
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography to ≥ 95% purity as determined by SDS-PAGE, reduced.
Form:Supplied as a liquid in 10mM Tris, pH 8.0, 5% BSA, 0.05% PEG, 0.02% sodium azide. Labeled with Colloidal Gold.