Coupling Ratio:~10-20 moles of biotin per mole gp160.
Specificity:Biotin gp160 binds strepavidin-AP/HRP in ELISA and Western ELISA.
Biological Activity:Biotin gp160 binds to solid phase CD4 as determined by capture ELISA using strepavidin-HRP as a secondary reagent.
Applications:Suitable for use in T-cell Research, ELISA and Western Blot. Other applications not tested.
Recommended Dilutions:Capture ELISA: 0.1-10ng, using 100ng of CD4 in solid phase
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
仕様
Size:50ug
Source Antigen:Recombinant, Baculovirus
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography to ≥ 95% purity as determined by SDS-PAGE, reduced.
Form:Supplied as a liquid in 0.1M Tris, 0.5% BSA, 0.05% tween-20, 0.01% Thimersol. Labeled with Biotin.