Gene cloned from Macrophage-cell-tropic HIV-1 YU2 strain and the full length (membrane anchor truncation) recombinant envelope gp160 glycoprotein produced in the Baculovirus Expression System.
Specificity:This protein binds to murine monoclonal antibodies of defined epitope specificity and HIV-1 converted human serum polyclonal antibodies in ELISA and Western ELISA.
Biological Activity:This protein binds to human T-cell receptor CD4 in ELISA and Western ELISA as determined by CD4/gp160/Anti gp160 mAb-peroxidase capture ELISA. Cell activation Assays pending.
Applications:Suitable for use in human diagnostics, CD4 binding, drug screening, human T-cell CD4 receptor binding and co-receptor binding studies. Other applications not tested
Recommended Dilutions:Western Blot: 10-100ngELISA: yields titers of 1:1000 or greater at 10-100ng of immobilized protein under standard ELISA conditions. Optimal dilutions to be determined by the researcher.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Source Antigen：Recombinant, Baculovirus
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography to ≥ 95% purity as determined by SDS-PAGE, reduced.
Form：Supplied as a liquid in PBS, pH 7.2. No stabilizers or preservatives added.
