Biological Activity:This FITC-conjugated gp120 binds to CD4 bearing cells in FACS.
Applications:Suitable for use in Direct Immunoflouorescent staining, FACS, Immunohistochemistry and Dot Blot. Other applications not tested.
Recommended Dilutions:Direct Immunofluorescence: 1:50Dot Blot: ≥1:100 using purified anti-gp120 monoclonal or polyclonal antibodies. Optimal dilutions to be determined by the researcher.
Storage and Stability:For long-term storage, aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable at -70°C for 6 months after receipt. Dilute required amount only prior to immediate use
Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50Tests
Source Antigen：Baculovirus derived recombinant gp120 from HIV-1 MN strain
Grade：Affinity Purified
Purity：Purified by gel exclusion chromatography and UF concentrated.
Form：Supplied as a liquid in 0.1M NaPO4, 50mM sodium chloride, pH 7.0. No stabilizers or preservatives added. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：This FITC-conjugated gp120 binds to murine monoclonal antibodies of defined epitope specificity and immobilized CD4 in Dot Blot assays.
