Gene cloned from T-cell-tropic HIV-1, MN strain and the full length recombinant envelope gp120 glycoprotein produced in the Baculovirus Expression System.
This protein binds to murine monoclonal antibodies of defined epitope specificity and human serum polyclonal antibodies in ELISA and Western Blot.
Biological Activity:This protein binds to human T-cell receptor CD4 in ELISA and Western ELISA as determined by CD4/gp120/Anti-gp120 mAb-peroxidase capture ELISA. This protein activates human T-Lymphocytes (CD4+, CD4-), in vitro, as measured by RNA synthesis during G0 to G1 transition phase of antigen-binding competent Cells.
Applications:Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:ELISA: 10-100ng protein depending on the nature and affinity of the detection reagent. HIV-converted human serum polyclonal antibodies yield titers of 1:1000 or greater at 1-10ng of immobilized protein under standard ELISA conditions.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.
仕様
Size：100ug
Source Antigen：Recombinant, Baculovirus
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography to ≥ 95% purity as determined by SDS-PAGE, reduced.
Form：Supplied as a liquid in PBS, pH 7.2. No stabilizers or preservatives added.
