Full length HIV-1 IIIB envelope gp120 cloned genes expressing oligomeric glycoprotein produced in the Baculovirus Expression System.
Specificity:This protein binds to murine monoclonal antibodies of defined epitope specificity and HIV-1 converted human serum polyclonal antibodies in ELISA and Western Blot.
Biological Activity:This protein binds to human T-cell receptor CD4 in ELISA and Western ELISA as determined by CD4/gp120/Anti-gp120 mAb-peroxidase capture ELISA. This protein activates human T-Lymphocytes (CD4+, CD4-), in vitro, as measured by RNA synthesis during G0 to G1 transition phase of antigen-binding competent cells.
Applications:Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions:Applications require 10-100ng protein depending on the nature and affinity of the detection reagent. Human serum polyclonal antibodies yield titers of 1:1000 or greater at 10-100ng of immobilized protein under standard ELISA conditions.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for 6 months after receipt.
仕様
Size：50ug
Source Antigen：Recombinant, Baculovirus
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography to ≥98% purity as determined by SDS-PAGE, non-reduced and reduced.
Form：Supplied as a liquid in PBS, pH 7.2. No stabilizers or preservatives added.
