Coupling Ratio:.~5-20 moles of biotin per mole gp120.
Specificity:Recognized by human and mouse anti-gp120 monoclonal antibodies.
Biological Activity:Biotin gp120 binds to solid phase CD4 as determined by capture ELISA using strepavidin-HRP as a secondary reagent.
Applications:Suitable for use in T-cell Research, ELISA and Western Blot. Other applications not tested.
Recommended Dilutions:ELISA: 1:1000Capture ELISA: 10-1000ng, using 100ng of CD4 in solid phase
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Recombinant
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography to ≥ 95% purity as determined by SDS-PAGE, reduced.
Form：Supplied as a liquid in PBS, 0.2% BSA, pH 7.5, 0.09% sodium azide. Labeled with Biotin.
