Molar Ratio:2-4 peroxidase molecules per molecule of gp120.
Specificity:Peroxidase conjugated rgp120 IIIB binds to murine monoclonal antibodies of defined epitope specificity and human monoclonal/polyclonal antibodies in ELISA and Western ELISA.
Biological Activity:Peroxidase conjugated rgp120 IIIB binds to immobilized human T-cell receptor CD4 in ELISA and Western ELISA.
Applications:A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. 1. ELISA and Western ELISA may be performed at dilutions of 1:1000 range depending on the concentration of CD4 in the test system. Binding to murine and human monoclonal/polyclonal antibodies may be performed at higher dilutions.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200Tests
Source Antigen：Recombinant, Baculovirus
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography to ≥ 95% purity as determined by SDS-PAGE, reduced.
Form：Supplied as a liquid in 50mM Tris, pH 8.0, 1% BSA, 0.01% Thimerosal. Labeled with HRP.
