Full length Recombinant HCV 70kD envelope-GST fusion protein produced in the Baculovirus Expression System.
Hepatitis C virus (HCV) is one of the leading causes of chronic liver diseases and B-lymphocyte proliferative disorders, including mixed cryoglobulinemia and B-cell lymphoma. It has been suggested that HCV infects human cells through the interaction of its envelope glycoprotein E2 with a tetraspanin molecule CD81, the putative viral receptor.
Recognizes murine monoclonal antibodies of defined epitope specificity and HCV converted human serum polyclonal antibodies in ELISA and Western Blot.
Applications:Suitable for use in ELISA, Western Blot, T-cell activation and Immunization. Other applications have not been tested.
Recommended Dilutions: ELISA: yields titers of 1:1000 or greater at 10-100ng of immobilized protein under standard ELISA conditions
Optimal dilutions to be determined by the researcher.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ug
Source Antigen:Recombinant, Baculovirus
Grade:Affinity Purified
Purity:Purified by glutathione affinity chromatography to ≥95% purity as determined by SDS-PAGE, reduced.
Form:Supplied as a liquid in PBS, pH 7.2. No stabilizers or preservatives added.