Applications:Suitable for use in ELISA, Western Blot, solid phase antigen-antibody titration assay and T-Cell Activation. Other applications not tested.
Recommended Dilutions:solid phase antigen-antibody titration assay:Dilute the antigen preparation 10 to 20-fold in PBS
Western Blot: 10ng range depending on the nature and affinity of the secondary detection reagent. ELISA: HCV converted human serum polyclonal antibodies yield titers of 1:10, 000 or greater at 100ng of immobilized protein under standard ELISA conditions
Optimal dilutions to be determined by the researcher.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Source Antigen：Recombinant, Yeast
Grade：Affinity Purified
Purity：~90% by SDS-PAGE, reduced. Purified by immunoaffinity chromatography methods.
Form：Supplied as a liquid in 50mM Tris, pH 8.0, 0.1mM sodium chloride. No stabilizers or preservatives added.
Specificity：This protein binds murine monoclonal antibodies and HCV converted human serum polyclonal antibodies in ELISA and Western Blot.
