Indirect immunofluorescence test for the detection of antibodies against Hantavirus (Puumala serotype) in rat or mouse serum.
Different serotypes of Hantavirus cause a complex of infectious diseases in humans known as Hemorrhagic Fever with Renal Syndrome (HFRS). Hantaviruses cause inapparent infection in rodents. Transmission from animals to humans occurs via virusinfected aerosols or via direct contact. In humans, Hantaan serotype (HTN) is the etiological agent of the severe form of HFRS and is predominantly found in Asia, Southeast and Mediterranean European countries. InScandinavia, Central Europe and the CIS, Puumala serotype (PUU) is responsible for a milder form of the disease, known as Nephropathia epidemica (NE). Another form of HFRS, found predominantly in urban regions (mainly in Asia) is caused by viruses of the Seoul serotype
However, the course of the disease is milder than with the Hantaan serotype. The initial diagnosis of a Hantavirus infection in humans is based on clinical symptoms and should be confirmed by serology. A few days after the onset of the disease IgM antibodies are detectable in human sera reaching peak titers after 2 to 3 weeks. IgG antibodies develop early andmay persist for years. There is a one-way cross-reactivity between Hantaan and Puumala serotypes: sera containing antibodies against Puumala serotype often react with Hantaan virus
Antibodies against the Seoul virus are often also detected with the Hantaan IF test. In recent years new serotypes were detected, e.g. Four Corners Virus in the USA, Dobrava/Belgrad in Slovenia. A limited number of sera tested showed that all Four Corners Virus sera reacted inthe Puumala IFA, sera from Dobrava-infected patients reacted with in the Hantaan Virus IFA.
60 testsReagent kit, 6 x 10-well slidesConjugate: anti-mouse + anti-rat IgG/IgM FITCAntigen: Seoul Virus-infected VeroE6 cellsIncubation: 30/30 min
Kit Components:H1810-30A Positive Control: Puumala Mab 1x200ulSupplied as a lyophilized powder, <0.1% sodium azide. Reconstitute with 200ul sterile ddH2O Stable at 4°C for 4 weeks
H1810-30B Positive Control: Seoul Mab 1x200ulSupplied as a lyophilized powder, <0.1% sodium azide. Reconstitute with 200ul sterile ddH2O Stable at 4°C for 4 weeks
H1810-30C Positive Control: Hantaan Mab 1x200ulSupplied as a lyophilized powder, <0.1% sodium azide. Reconstitute with 200ul sterile ddH2O Stable at 4°C for 4 weeks
H1810-30D IgG and IgM (Cy™2) Pab goat anti-rat/mouse 1x2ml Supplied as a lyophilized powder, <0.1% sodium azide and Evans Blue. Reconstitute with 2ml sterile ddH2O Stable at 4°C for 4 weeks
The conjugate contains Evans Blue (toxic, carcinogenic, terato-genic). Do not swallow! Avoid any contact with skin or mucous membranes!H1810-30E Buffered glycerin mounting medium 1x3ml Supplied as a liquid, <0.1% sodium azide
H1810-30F PBS 2x1L Supplied as a lyophilized powder. Reconstitute the contents of each container with 1L sterile ddH2O
H1810-30G Hantavirus Puumala Slides 6x1 slides (10 wells per slide) with fixed infected and non-infected cells. Although fixed, slides should be handled as potentially infectious.
Storage and Stability:Store at 4°C. Stable for 4 weeks. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
