Desmin is the main intermediate filament in mature skeletal, cardiac and smooth-muscle cells. Can be used to distinguish rhabdomyosarcomas and leiomyosarcomas from other soft tissue tumors, lymphomas and carcinomas.
Molecular Weight:Calculated from sequence: 53.5396kDObserved (SDS Gel Electrophoresis): 55kD
Isoelectric Point:pI 5.16 (calculated from sequence).
Applications:Suitable for use as protein standard in 1D and 2D SDS gel electrophoresis, in Immunoassays and in immunization. Other applications not tested.
Reconstitution to Filaments:Protofilaments and filament complexes are obtained by dialyzing the reconstituted polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50mM sodium chloride, 2mM DTT, 10mM Tris-HCl, pH 7.4)
For Use in Immunization:The solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS).
Storage and StabilityStore lyophilized product at -20°C. Store reconstituted product in aliquots at -20°C to avoid repeated freeze-thaw cycles. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：≥95% (determined by SDS gel electrophoresis).
Form：Supplied as a lyophilized powder. Reconstitute with 70ul sterile water. Final Solution contains: 30mM Tris-HCI, pH 8, 9.5M urea, 2mM DTT, 2mM EDTA, 10mM methylammonium chloride.
