85-1440-33　［取扱停止］Vimentin, Recombinant, Human (FLJ36605, VIM) 100ug　V2122-15

特徴

• Vimentin expression in human malignant glioma cells depends on cellular density, algorithms of drug delivery and chemo/radio treatment. Vimentin and detyrosinated microtubules provide structural support for the extensive microtentacles observed in detached tumor cells and a mechanism to promote successful metastic spread. Primary colorectal carcinomas display aberrant expression of Vimentin, and have activated Notch and TGF-beta signaling pathways. Vimentin is a strong arterial substrate for transglutaminases. Transglutaminase-mediated Vimentin dimerization results in a novel unifying pathway by which vasodilatory and remodeling responses may be regulated. Ablation of Vimentin expression inhibits migraion and invasion of colon and breast cancer cell lines. Vimentin is the main intermediate filament protein in mesenchymal cells and is therefore of value in the differential diagnosis of undifferentiated neoplasms.
• Recombinant Human Vimentin produced in E. coli is a non-glycosylated, polypeptide chain. On SDS-PAGE commassie blue stained gel, the purified recombinant protein shows a band at 57kD.
• Reconstitution to Filaments:After Vimentin is dissolved, protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50mM sodium chloride, 2mM dithiothreitol, 10mM Tris-HCl, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco’s PBS). (7,8)
• Applications:Suitable for use in ELISA, Western Blot and Inhibition Studies. Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.