85-1440-22　［取扱停止］Leptin, Triple Antagonist, Recombinant, Human (Ob, Obese Protein, Obesity Factor) 10ug　L1670-15C

特徴

• Leptin inhibits food intake and stimulates energy expenditure. Leptin also has thermogenic actions and regulates enzymes of fatty acid oxidation. Severe hereditary obesity in rodents and humans is caused by defects in leptin production. In addition to its critical role in the physiologic regulation of body weight leptin has a variety of other physiologic and pathologic functions resembling those of cytokines. These functions include the regulation of hematopoiesis, angiogenesis, wound healing, inflammation, and immune responses.
• Recombinant human leptin, one polypeptide chain containing 146 amino acids and additional Ala at N-terminus and having a molecular mass of ~16kD, hLEP was mutated, resulting in L39A/D40A/F41A mutant was purified by proprietary chromatographic techniques.
• Amino Acid Sequence:The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-Gln
• Dimers and Aggregates:The purified hLEP triple antagonist (16K) consists of > 95% monomers as determined by gel-filtration chromatography.
• Endotoxin:Less than 0.1 ng/ug (IEU/ug) of human LEP triple antagonist
• Biological Activity:HumanLEP triple antagonist is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. It also inhibits various leptin effects in several in vitro bioassays.
• Protein Content:Protein quantitation was carried out by UV spectroscopy at 280nm using the absorbency value of 0.88 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
• Solubility:It is recommended to reconstitute the lyophilized hLEP mutant in sterile 0.4% NaHCO3 adjusted to pH 8-9, not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 0.4% NaHCO3 and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.