17-aa peptide from Mouse OCTN2 (1). Corresponding antibodies (N5376-54, N5376-54A) recognize human, mouse and rat Novel Organic Cation Transporter 2. The 17aa mouse control peptide is 88% conserved in rat, 76% in human OCTN2. No significant sequence homology is detected with other OCTs or OCTNs.
Mammalian kidney and liver are critical in maintaining physiological ionic environment. Kidney specializes in removing toxins, drugs, and other organic cations from the blood by an active process called "renal secretion". Functional studies have identified two distinct categories of organic cation transporters (OCTs): a system driven by transmembrane potential difference that governs the influx of cations, whereas the H+-gradient-dependent transport system may mediate the efflux of organic cations. Several multispecific, potential-sensitive transporters (OCT1-3) and H+-dependent transporters (OCTN1-3) have been cloned and characterized from various tissues. OCT superfamily of proteins shares high degree of sequence homology, display up to 12 transmembrane domains with cytoplasmic N and C-terminus.
OCTN2, a structural homolog of OCTN1, has been identified as carnitine transporter. OCTN2 (human/rat/mouse 557 aa) shares ~76% homology with OCTN1. It is strongly expressed in human kidney, skeletal muscle, heart, and placental in adult humans. In rat, OCTN2 expression was found in the proximal and distal tubules and in the glomeruli in the kidney, in the myocardium, valves, and arterioles in the heart, in the labyrinthine layer of the placenta, and brain (cortex, hippocampus, and cerebellum). OCTN2 mediated uptake of carnitine in a Na+-dependent manner, whereas other organic cations were transported without Na+.
Applications:Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:ELISA: 5-10ug control peptide per 1ug of aff pure IgG or 1ul antiserum to confirm antibody specificity Primary antibody 1:10,000-1:100,000
Optimal dilutions to be determined by the researcher. The control peptide is available to confirm specificity of antibodies. Control peptide, because of its low MW (<3kD), is not suitable for Western. It should be used for ELISA or antibody blocking experiments.
Location: Cytoplasmic C-terminus
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Grade：Highly Purified
Purity：Highly Purified
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
