HtrA2/Omi is a mammalian serine protease at high temperatures and a chaperone at low temperature. The full-length HtrA2 is synthesized as a precursor protein and then targeted to the mitochondria where it is matured by the removal of N-terminal 133 residues. Mature HtrA2 consists of a putative transmembrane domain; an inhibitor of apoptosis protein (IAP)-binding motif; a single C-terminal PDZ domain that mediates protein-protein interactions. Recently, HtrA2 has known to contribute both to caspase-dependent and caspase-independent cell death.
Htra2 is a novel member of the Htra serine protease family. Human Htra2 is localized in the mitochondria and is involved in mammalian stress response pathways. Human Htra2, predominantly a nuclear protease, is upregulated in mammalian cells under stress, due to heat shock or tunicamycin treatment. Htra2 plays a role in regulating apoptosis by binding and inhibiting caspases.
The inhibitor of apoptosis (IAP) family of proteins regulates programmed cell death triggered by various stimuli. All IAPs have at least one baculovirus IAP repeat (BIR) motif that is essential for their anti-apoptotic activity (1-3). Recently, a serine protease has been isolated, which is released from the mitochondria upon induction of apoptosis by apoptotic stimuli. This protein called Omi/HtrA2 is a mitochondrial protein that binds to IAP. Like Smac/DAIBLO, the mature Omi protein contains a conserved IAP-binding motif (AVPS) at its N-terminus. The deregulated expression of Omi in the cytoplasm of mammalian cells induces apoptosis indicating that Omi could participate in the mitochondrial apoptotic pathway.
Location:15aa near the C-terminus of human OMI (1).
Applications:The peptide is used for blocking the activity of OMI (Htr2A) antibody. It usually blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37°C.
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, pH 7.2, 0.1% BSA, 0.02% sodium azide.
